BIO-8104 | UiT

Obligatory prerequisites

BIO-2601 General microbiology, MBI-1001 Cell and molecular biology, MBI-2001 Biochemistry

Course overlap

If you pass the examination in this course, you will get an reduction in credits (as stated below), if you previously have passed the following courses:

BIO-3134 Applied Molecular Genetics 20 stp

Course content

The aim with this course is to give a thorough introduction to molecular genetic methods used in modern biological research. All lectures will be closely connected to the practical problems to be solved in the laboratory. Written laboratory reports will be subjects for plenary discussions as well as literature seminars and a mandatory report. The course will give a basis for advanced studies in different areas of microbiology and will therefore contain problems concerning medicine, ecology and food-microbiology.

Four practical problems are planned within the following topics: Taxonomy, genetic diversity, identification and characterisation, molecular microbal ecology and horizontal gene transfer.

The following methods will be used: rep-PCR-fingerprinting, 16S-RFLP, fluorescence in situ hybridisation (FISH), microarray, real-time PCR, Pulsed field gel electrophoresis (PFGE), conjugation and transformation. The teaching will also address bioinformatic tools for phylogeny and microarray technology.

Recommended prerequisites

MBI-2002 Molecular cellbiology

Language of instruction and examination

The language of instruction is English and all of the syllabus material is in English. Examination questions will be given in English, but may be answered either in English or a Scandinavian language.

Teaching methods

Lectures 10 hours, field and laboratory exercises 60 hours, seminars and project work 40 hours.

Assessment

Laboratory report, seminars based on submitted written material from group work, graded project essay, 4 h written exam with grades. Letter grades, A-F.

Error rendering component

autumn 2012 BIO-8104 Applied molecular genetics - 20 ECTS