Publications

2013

Cazaméa-Catalan, D., Magnanou, E., Helland, R., Besseau, L., Boeuf, G., Falcón, J., Jørgensen, E.H. (2013) Unique arylalkylamine N-acetyltransferase-2 polymorphism in Salmonids and profound variations in thermal stability and catalytic efficiency conferred by two residues. J Exp Biol 216, 1938-1948

Harmsen, R.A.G., Sivertsen, A., Michetti, D., Brandsdal, B.O., Sydnes, L.K., Haug, B.E. (2013) Synthesis and docking of novel piperidine renin inhibitors. Monatsh Chem In press

Borra, P.S., Samuelsen, O., Spencer, J., Walsh, T.R., Lorentzen, M.S., Leiros, H.K. (2013) Crystal structures of Pseudomonas aeruginosa GIM-1: active site plasticity in metallo-β-lactamases. Antimicrob Agents Chemother 57(2), 848-854

Fu, J., Leiros, H.K., de Pascale, D., Johnson, K.A., Blencke, H.M., Landfald, B. (2012) Functional and structural studies of a novel cold-adapted esterase from an Arctic intertidal metagenomic library. Appl Microbiol Biotechnol 97(9), 3965-3978

2012

Karstad, R., Isaksen, G., Wynendaele, E., Guttormsen, Y., De Spiegeleer, B., Brandsdal, B.O., Svendsen, J.S., Svenson, J. (2012) Targeting the S1 and S3 subsite of trypsin with unnatural cationic amino acids generates antimicrobial peptides with potential for oral administration. J Med Chem. 55(14), 6294-6305

Ahmad, R., Hansen, G.Å., Hansen, H., Hjerde, E., Pedersen, H.L., Paulsen, S.M., Nyrud, M.L.J., Willassen, N.P., Haugen, P. (2012) Prediction, microarray and Northern blot analyses identify new intergenic small RNAs in Aliivibrio salmonicida. J. Mol. Micro. Biotech. 22(6), 352-360

Kahlke, T., Steinar Thorvaldsen, S. (2012) Molecular characterization of cold adaptation of membrane proteins in the Vibrionaceae core-genome. PLoS ONE. 7(12), e51761

Hanssen, K.Ø., Andersen, J.H., Stiberg, T, Engh, R.A., Svenson, J., Genevière, A.M., Hansen, E. (2012) Antitumoral and Mechanistic Studies of Ianthelline Isolated from the Arctic Sponge Stryphnus fortis. Anticancer Res 32 (10), 4287-4297

Åberg, E., Lund, B.A., Pflug, A., Gani, O., Rothweiler, U., de Oliveira, T.M., Engh, R.A (2012) Structural origins of AGC protein kinase inhibitor selectivities: PKA as a drug discovery tool. Biol Chem. 393 (10), 1121-1129

Lagos, L.X., Iliev, D.B., Helland, R., Rosemblatt, M., Jørgensen, J.B. (2012) CD40L - a costimulatory molecule involved in the maturation of antigen presenting cells in Atlantic salmon (Salmo salar) Dev Comp Immunol. 38, 416-430

Ve, T., Mathisen, K., Helland, R., Karlsen, O.A., Fjellbirkeland, A., Røhr, Å.K., Andersson, K.K., Pedersen, R.B., Lillehaug, J.R., Jensen, H.B. (2012) The Methylococcus capsulatus (Bath) secreted protein, MopE*, binds both reduced and oxidized copper. PLoS ONE. 7, e43146

Lindell, K., Fahlgren, A., Hjerde, E., Willassen, N.P., Fällman, M., Milton, D.L. (2012) Lipopolysaccharide O-antigen prevents phagocytosis of Vibrio anguillarum by rainbow trout (Oncorhynchus mykiss) skin epithelial cells. PLoS One 7(5, e37678

Fu, J., Leiros, H.K., de Pascale, D., Johnson, K.A., Blencke, H.M., Landfald, B. (2012) Functional and structural studies of a novel cold-adapted esterase from an Arctic intertidal metagenomic library. Appl Microbiol Biotechnol In press

Leiros, H.K.S., Fedøy, A.E., Leiros, I., Steen, I.H. (2012) The complex structures of Isocitrate dehydrogenase from Clostridium thermocellum and Desulfotalea psychrophila, suggest a new active site locking mechanism. FEBS Open Bio. 2, 159-171

Cazaméa-Catalan, D., Magnanou, E., Helland, R., Vanegas, G., Besseau, L., Boeuf, G., Paulin, C.H., Jørgensen, E.H., Falcón, J. (2012) Functional diversity of Teleost arylalkylamine N-acetyltransferase-2: Is the timezyme evolution driven by habitat temperature? Mol Ecol. 21, 5027-5041.

Pflug, A., Johnson, K.A., Engh, R.A. (2012) Anomalous dispersion analysis of inhibitor flexibility - A case study of the kinase inhibitor H-89. Acta Cryst F68, 873-877 

Leiros, H.K.S., Borra, P.S., Brandsdal, B.O., Edvardsen, K.S.W., Spencer, J., Walsh, T.R., Samuelsen, Ø. (2012) Crystal Structure of the Mobile Metallo-b-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the role of Gln157. Antimicrob Agents Chemoter. 56, 4341-4353

Kahlke, T., Goesmann, A., Hjerde, E., Willassen, N.P., Haugen, P. (2012) Unique core genomes of the bacterial family vibrionaceae: Insights into niche adaptation and speciation. BMC Genomics, 13 (1), 179

Bjelland, A.M., Sørum, H., Tegegne, D.A., Winther-Larsen, H.C., Willassen, N.P., Hansen, H. (2012) LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence. Infect immune, 80 (5), 1681-1689

Bjornsdottir, B., Hjerde, E., Bragason, B.T., Gudmundsdottir, T., Willassen, N.P., Gudmundsdottir, B.K. (2012) Identification of type VI secretion systems in Moritella viscosa. Vet. Microbiol. 158, 436-442.

Moe, E., Hall, D.R., Leiros, I., Monsen, V.T., Timmins, J., McSweeney, S. (2012) Structure/function studies of an unusual 3-methyladenine DNA glycosylase II (AlkA) from Deinococcus radiodurans. Acta Cryst D68, 703-712.

Hansen GA, Ahmad R, Hjerde E, Fenton CG, Willassen NP, Haugen P. (2012) Expression profiling reveals Spot 42 small RNA as a key regulator in the central metabolism of Aliivibrio salmonicida. BMC Genomics. 13, 37-49.

McManus, H.A., Lewis, L.A., Fučíková, K., Haugen, P. (2012). Invasion of protein coding genes by green algal ribosomal group I introns. Molecular Phylogenetics and Evolution. 62(1), 109-116.

Assefa, N.G., Niiranen, L., Willassen, N.P., Smalås, A.O., Moe, E (2012) Thermal Unfolding Studies of Cold Adapted Uracil-DNA N-Glycosylase (UNG) From Atlantic Cod (Gadus morhua). A Comparative Study with Human UNG. Comp Biochem Physiol B, 161(1),60-68.

Karlsen C, Sørum H, Willassen NP, Åsakk K (2012) Moritella viscosa bypasses Atlantic salmon epidermal keratocyte clearing activity and might use skin surfaces as a port of infection. Vet Microbiol. 154(3-4), 353-362.

Gruber FX, Ernst T, Porkka K, Engh RA, Mikkola I, Maier J, Lange T, Hochhaus A. (2012) Dynamics of the emergence of dasatinib and nilotinib resistance in imatinib-resistant CML patients. Leukemia 26, 172-177.

2011
Weber B., Lindell K., Elqquaidi S., Hjerde E., Willassen N. P. and Milton D. L. The phosphotransferase VanU represses expression of four qrr genes antagonizing VanO-mediated quorum-sensing regulation in Vibrio anguillarum. Microbiology, 157, 3324-3339.

Karlsen C, Espelid S, Willassen NP, Paulsen SM (2011) Identification and cloning of immunogenic Aliivibrio salmonicida Pal-like protein present in profiled outer membrane and secreted subproteome. Dis Aquat Org 93, 215-223.

Pflug, A., de Oliveira, T.M., Bossemeyer, D., Engh, R.A. (2011) "Mutants of protein kinase A that mimic the ATP-binding site of Aurora kinase". Biochem J. 440, 85-93.

Rothweiler, U., Åberg, E., Johnson, K.A., Hansen, T.E., Jørgensen, J.B., and Engh, R.A. (2011) "p38α MAP Kinase Dimers with Swapped Activation Segments and a Novel Catalytic Loop Conformation". J Mol Biol 411, 474-48.

Borra, P.S., Leiros, H.K., Ahmad, R., Spencer, J., Leiros, I., Walsh, T.R., Sundsfjord, A., Samuelsen, O. (2011) "Structural and computational investigations of VIM-7: Insights into the substrate specificity of VIM  metallo-beta-lactamases" J Mol Biol 411, 174-189.

Oliveira, T.M., Ahmad, R., Engh, R.A (2011) "Vx680 binding interactions with the glycine rich loop aromat of Aurora A". J. Phys. Chem. A 115, 3895-3904.

M.E. Prime, M.E., Courtney, S.M., Brookfield, F.A.,  Marston, R.W.,  Walker, V.,  Warne, J.,  Boyd, A.E.,  Kairies, N.A.,  von der Saal, W., Limberg,  A., Georges, G.,  Engh, R.A.,  Goller, B.,  Rueger, P.,  Rueth, M. (2011) "Phthalazinone pyrazoles as potent, selective and orally bioavailable inhibitors of Aurora-A kinase". J. Med. Chem  54, 312-319

von Bubnoff, N., Gorantla, S.P., Engh, R.A., de Oliveira, T., Thöne, S., Åberg, E., Peschel, C., Duyster, J. (2011) "The low frequency of clinical resistance to PDGFR inhibitors in myeloid neoplasms with abnormalities of PDGFRA might be related to the limited repertoire of possible PDGFRA kinase domain mutations in vitro. " Oncogene, 30, 933-943.

2010
Pedersen HL, Hjerde E, Paulsen SM, Hansen H, Olsen L, Thode SK, Dos Santos MT, Paulssen RH, Willassen NP, Haugen P. (2010) Global responses of Aliivibrio salmonicida to hydrogen peroxide as revealed by microarray analysis. Marine Genomics, 3, 193-200.

Janowski, S., Kormeier, B., Töpel, T., Hippe, K., Hofestädt, R,. Willassen, N.P., Friesen, R., Rubert, S., Borck, D., Haugen, P., Chen, M (2010) "Modeling of cell-cell communication processes with Petri nets using the example of quorum sensing" In Silico Biology. 10, 0003.

Knævelsrud, I., Moen, M.N., Grøsvik, K., Haugland, G.T., Birkeland, N.K., Klungland, A., Leiros, I., Bjelland, S. (2010) "The Hyperthermophilic Euryarchaeon Archaeoglobus fulgidus Repairs Uracil by Single Nucleotide Replacement. " J Bacteriol. 192 (21), 5755-5766

Byeon, I.J., Dao, K.K., Jung, J., Keen, J., Leiros, I., Døskeland, S.O., Martinez, A. & Gronenborn, A.M. (2010) "Allosteric communication between cAMP binding sites in the RI subunit of protein kinase A revealed by NMR. " J Biol Chem. 285(18), 14062-14070

Pflug, A., Rogozina, J., Lavogina, D., Enkvist, E., Uri, A., Engh, R.A., Bossemeyer, D. (2010) "Diversity of Bisubstrate Binding Modes of Adenosine Analogue-Oligoarginine Conjugates in Protein Kinase A and Implications for Protein Substrate Interactions." J Mol Biol. 403 (1), 66-77

Karstad, R., Isaksen, G., Brandsdal, B.O., Svendsen, J.S., Svenson, J. (2010) "Unnatural Amino Acid Side Chains as S1, S1', and S2' Probes Yield Cationic Antimicrobial Peptides with Stability toward Chymotryptic Degradation" J Med Chem B. 53 (15), 5558-5566

Mereghetti, P., Riccardi, L., Fantucci, P., Brandsdal, B.O., De Gioia, L. and Papaleo, E. (2010) "Near Native-State Free Energy Landscape of Psychrophilic and Mesophilic Enzymes: Probing the Folding Funnel Model" J Phys Chem B. 114 (22), 7609-7619

Kyomyhendo, P., Myrnes, B., Brandsdal, B.O., Smalås, A.O., Nilsen, I.W., Helland, R. (2010) "Thermodynamics and structure of a salmon cold-active goose-type lysozyme." Comp Biochem Biophys B. . 156, 254-263

Gani, O. A.B.S.M. and Engh, R.A. (2010) "Protein kinase inhibition of clinically important staurosporine analogues". Natural Product Reports. 27 (4), 489-498.

Leiros, H.-K.S., Brandsdal, B.O. & McSweeney, S.M. (2010). "Biophysical characterization and mutational analysis of the antibiotic resistance protein NimA from Deinococcus radiodurans." Biochim Biophys Acta-Proteins and Proteomics. 1804 (4), 967-976

Ræder, I.L.U., Moe, E., Willassen, N.P., Smalås, A.O., Leiros, I. (2010) "Structure of uracil-DNA N-glycosylase (UNG) from Vibrio cholerae: mapping temperature adaptation through structural and mutational analysis. " Acta Cryst. F66, 130-136.

Pedersen, H.L., Ahmad, R., Riise E.K., Leiros, H.-K.L., Hauglid, S., Espelid, S., Brandsdal, B.O., Leiros, I, Willassen, N.P., Haugen, P. (2010). "Experimental and Computational Characterisation of the Ferric Uptake Regulator from Aliivibrio salmonicida (Vibrio salmonicida) ". The Journal of Microbiology. 48, 174-183.

2009
von Bubnoff N, Engh RA, Aberg E, Sänger J, Peschel C and Duyster J. (2009) "FMS-like tyrosine kinase 3 internal tandem duplication tyrosine kinase inhibitors display a nonoverlapping profile of resistance mutations in vitro", Cancer Res. 69, 3032-41.

Pflug, A., de Oliveira T.M., Bossemeyer D. and Engh, R.A. (2009) "Mutants of protein kinase A with Aurora kinase inhibitor specificity", Acta Biochemica Polonica S1, 66-67.

Helland, R., Larsen, R.L., Finstad, S., Kyomuhendo, P., Larsen, A.N. (2009) "Crystal structures of g-type lysozyme from Atlantic cod shed new light on substrate binding and the catalytic mechanism." Cell Mol Life Sci. 66 ,2585-2598.

Svenson,J., Karstad, K., Flaten, G.E., Brandsdal, B.O., Brandl, M. and Svendsen, J.S. (2009) "Altered Activity and Physicochemical Properties of Short Cationic Antimicrobial Peptides by Incorporation of Arginine Analogues." Mol Pharmaceutics, 6, 996-1005.

Knaevelsrud, I., Slupphaug, G., Leiros, I., Matsuda, A., Ruoff, P., Bjelland, S. (2009) "Opposite-base dependent excision of 5-formyluracil from DNA by hSMUG1." Int J Radiat Biol 85 ,413-420.

Robertson, A.B., Klungland, A., Rognes, T., Leiros, I.(2009) "DNA repair in mammalian cells: Base excision repair: the long and short of it." Cell Mol Life Sci. 66 ,981-93.

Pedersen, H.L., Willassen, N.P., Leiros, I. (2009) "The first crystal structure of a cold-adapted superoxide dismutase (SOD). Biochemical and structural characterisation of iron SOD from Vibrio salmonicida." Acta Cryst, F65, 84-92.

Hjerde, E., Lorentzen, M.S., Holden, M.T., Seeger, K., Paulsen, S., Bason, N., Churcher, C., Harris, D., Norbertczak, H., Quail, M.A., Sanders, S., Thurston, S., Parkhill, J., Willassen, N.P., Thomson, N.R. (2009) The Genome Sequence Of The Fish Pathogen Aliivibrio salmonicida Strain LFI1238 shows extensive evidence of gene decay, BMC Genomics, 9, 616

Ahmad, R., Brandsdal, B.O., Michaud-Soret, I. and Willassen, N.P. (2009) "Ferric uptake regulator protein: Binding free energy calculations and per-residue free energy decomposition." Proteins, 75 373-386.

Ahmad, R., Hjerde, E., Hansen, G.Å., Haugen, P. & Willassen, N.P. (2009) "Prediction and experimental testing of Fur regulons in vibrios." J. Mol. Microbiol. Biotechnol. 16 159-168.

Helland, R., Larsen, R.L. and Asgeirsson, B. (2009) "The 1.4 Å crystal structure of the large and cold-active Vibrio sp. alkaline phosphatase." BBA-Proteins and Proteomics,1794, 297-308.

Szlachcic,A., Zakrzewska, M., Krowarsch, D., Vibeke Os, V., Helland, R., Smalås, A.O. and Otlewski, J. (2009) “Structure of a highly stable mutant of human fibroblast growth factor 1”. Acta Cryst D, 65, 67-73.

2008
Engh, R. (2008) Protein kinase inhibitors highlight the complexities of drug target noncovalent interactions, Biotechnol. & Biotechnol. Eq., 22, 773-777.

Bjelic, S., Brandsdal, B.O. and Åqvist, J. (2008) "Cold-Adaptation of Enzyme Reaction Rates." Biochemistry 47(38), 10049-10057.

Karlsen, C., Paulsen, S.M., Tunsjø, H.S., Krinner, S., Sørum, H., Haugen, P., Willassen, N.P. (2008) ”Motility and flagellin gene expression in the fish pathogen Vibrio salmonicida: Effects of salinity and temperature.” Microb Pathog. 45(4), 258-264.

Strandskog, G., Skjæveland, I., Ellingsen, T., Jørgensen, J.B.(2008) "Double-stranded RNA- and CpG DNA-induced immune responses in Atlantic salmon: Comparison and synergies". Vaccine. 26(36), 4704-4715.

Skjæveland, I., Iliev, D.B., Zou, J., Jørgensen, T., Jørgensen, J.B. (2008) "A TLR9 homolog that is up-regulated by IFN-gamma in Atlantic salmon (Salmo salar)". Dev Comp Immunol. 32(6), 603-607.

Koutsioulis, D., Wang, E., Tzanodaskalaki, M., Nikiforaki,, D., Deli, A., Feller, G., Heikinheimo, P., Bouriotis V.. (2008), "Directed evolution on the cold adapted properties of TAB5 alkaline phosphatase." Protein Eng Des Sel. 21, 319-327.

Kapp,U. Macedo,S., Hall, D., Leiros,I., S. McSweeney, S. and Mitchell, E. (2008) "Crystal structure of Deinococcus radiodurans tunicamycin resistance protein (TmrD): a phosphotransferase." Acta Cryst F,, 64, 479-486.

Leiros, H.-K. S., Tedesco, C. and McSweeney, S. (2008) "High resolution crystal structure of the antibiotic resistance protein NimA from Deinococcus radiodurans." Acta Cryst F., 64, 442-447.

Helland, R, Fjellbirkeland, A, Karlsen, OA, Ve, T, Lillehaug, JR & Jensen, HB (2008). "An oxidized tryptophan facilitates copper binding in Methylococcus capsulatus-secreted protein MopE." J. Biol. Chem., 283, 13897-13904.

Niiranen, L., Altermark, B., Brandsdal, B.O., Leiros, H.K.S., Helland, R., Smalås, A.O. and Willassen, N.P. (2008) "Effects of salt on kinetics and thermodynamic stability of endonuclease I from Vibrio salmonicida and Vibrio cholerae." FEBS J., 275, 1593-1605.

Kyomohendo, P., Nilsen, I.W., Brandsdal, B.O. & Smalås, A.O. (2008) "Inhibition of marine g-type lysozymes by invertebrate inhibitor of lysozyme." J. Mol. Mod., 14 , 777-788.

Svenson, J., Stensen, W., Brandsdal, B.O., Haug, B.E., Monrad, J. & Svendsen, J.S. (2008) "Antimicrobial peptides with stability towards tryptic degradation." Biochemistry., 47 ,3777-3788.

Olufsen, M., Papaleo, E., Smalås, A.O. & Brandsdal, B.O. (2008). "Ion pairs and their role in modulating stability of cold- and warm-active uracil DNA glycosylase." Proteins. 71 , 1219-1230.

Olufsen, M., Smalås, A.O. & Brandsdal, B.O. (2008) "Electrostatic interactions play an essential role in DNA repair and cold-adaptation of Uracil DNA glycosylase." J. Mol. Mod., 14 , 201-213.

Altermark, B., Helland, R., Moe, E., Willassen, N.P. & Smalås, A.O. (2008) "Environmental adaptation of endonuclease I from Vibrio salmonicida." Acta Cryst D., 64, 368-376

2007
Timmins, J., Leiros, I. & McSweeney, S. (2007) "Crystal structure and mutational study of RecOR provide insight into its mode of DNA binding." EMBO Journal., 26, 3260-3271

Solstad, T., Stenvik, J. & Jørgensen, T.Ø. (2007) "mRNA expression patterns of the BPI/LBP molecule in the Atlantic cod (Gadus morhua L.)." Fish & Shellfish Immunology, 23, 260-271.

Fedøy, A.-E., Yang, N., Martinez, A., Leiros, H.-K.S. & Steen, I.H. (2007). "Structural and functional properties of isocitrate dehydrogenase from the psychrophilic bacterium Desulfotalea psychrophila." J. Mol Biol., 372, 130-49.

Leiros, H-K., Pey, A., Innselset, M., Moe, E., Leiros, I., Steen, IH. & Martinez, A. (2007) "Structure of phenylalanine hydroxylase from Colwellia psychrerythraea 34H, a monomeric cold active enzyme with local flexibility around the active site and high overall stability." J. Biol. Chem., 282, 21973-21986.

Svenson, J., Brandsdal, B.O., Stensen, W. & Svendsen, J.S. (2007) "Albumin binding of short cationic antimicrobial micropeptides and its influence on the in vitro bactericidal effect." J. Med. Chem., 50, 3334-3339.

Thorvaldsen, S., Hjerde, E., Fenton, C. and Willassen, N.P. (2007)"Molecular characterization of cold adaptation based on ortholog protein sequences from Vibrionaceae species." Extremophiles, 11, 719-732.

Sælensminde, G., Halskau jr., Ø., Helland, R., Willassen, N.P., and Jonassen, I. (2007) "Structure-dependent relationships between growth temperature of prokaryotes and the amino acid frequency in their proteins." Extremophiles 11, 585-596.

Olufsen, M., Brandsdal, B.O. and Smalås, A.O. (2007) "Comparative unfolding studies of psychrophilic and mesophilic uracil DNA glycosylase: MD simulations show reduced thermal stability of the cold-adapted enzyme." J. Mol. Graph. Mod. 26, 124-134.

Papaleo, E., Olufsen, M., de Gioia, L. and Brandsdal, B.O. (2007). "Optimization of electrostatics as a strategy for cold-adaptation: A case study of cold- and warm- active elastases." J. Mol. Graph. Mod. 26, 93-103.

Stokke R, Madern D, Fedoy AE, Karlsen S, Birkeland NK, & Steen IH. (2007) "Biochemical characterization of isocitrate dehydrogenase from Methylococcus capsulatus reveals a unique NAD(+)-dependent homotetrameric enzyme." Arch. Microbiol. 187, 361-370.

Altermark, B., Torvaldsen S., Moe, E., Smalås, A.O., & Willassen, N.P. (2007). "Sequence comparison and environmental adaptation of a bacterial endonuclease." Comput. Biol. Chem. 31, 163-172.

Smith Tunsjø, H., Paulsen, S.M., Mikkelsen, H., L'Abée-Lund, T., Skjerve, E. and Sørum, Henning. (2007) "Adaptive response to environmental changes in the fish pathogen Moritella viscose." Res. Microbiol. 158, 244-250.

Hansen, T.E. & Jørgensen, J.B. (2007) "Cloning and characterisation of p38 MAP kinase from Atlantic salmon. A kinase important for regulating salmon TNF-2 and IL-1β expression." Molecular Immunology. 44,3137-3146.

Stokke, R., Karlström, M., Yang, N., Leiros, I., Ladenstein, R., Birkeland, N-K. & Steen, IH. (2007) "Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers." Extremophiles. 11, 481-493.

Leiros, I., Nabong, MP., Grøsvik, K., Ringvoll, J., Haugland, GT., Uldal, L., Reite, K., Olsbu, IK., Knævelsrud, I., Moe, E., Andersen, OA., Birkeland, N-K., Ruoff, P., Klungland, A. & Bjelland, S. (2007) "Structural basis for enzymatic excision of N1-methyladenine and N3-methylcytosine from DNA." EMBO J. 26, 2206-2217.

Leiros, H.K.S. & McSweeney, S. (2007) "The crystal structure of DR2241 from Deinococcus radiodurans at 1.9 Å resolution reveals a multi-domain protein with structural similarity to chelatases but also with two additional novel domains." J. Struct. Biol., 159, 92-102.

Wang, E., Koutsioulis, D., Leiros, H-K. S, Andersen, O.A., Hough, E., & Heikinheimo, P. (2007) "The Crystal Structure of Alkaline Phosphatase from the Antarctic strain TAB5." J. Mol. Biol. 366, 1318-1331.

Niiranen L., Espelid, S., Karlsen, C.R., Mustonen, M., Paulsen, S.M., Heikinheimo, P. and Willassen, N.P. (2007) "Comparative expression study to increase the solubility of cold adapted Vibrio proteins in Escherichia coli." Protein Expression and purification, 52, 210-218.

Altermark, B., Niiranen, L., Willassen, N.P., Smalås, A.O., and Moe, E. (2007). "Comparative studies of endonuclease I from cold adapted Vibrio salmonicida and mesophilic Vibrio cholerae." FEBS Journal. 274, 252-263.

Riise, E. K., Lorentzen, M. S., Helland, R., Smalås, A.O., Leiros, H-K. and Willassen, N. P. (2007) "The first crystal structure of a cold active catalase from Vibrio salmonicida at 1.96 Å reveals structural aspects of cold adaptation" Acta Cryst D. D63, 135-148.

Ræder, I.L.U, Paulsen, S.M., Smalås, A.O. and Willassen, N.P. (2007). "Effect of fish skin mucus on the soluble proteome of Vibrio salmonicida analysed by 2-D gel electrophoresis and tandem mass spectrometry."Microbial Pathogenesis. 42, 37-46.

2006
Leiros, I., Wang, E., Rasmussen, T., Oksanen, E., Repo, H., Petersen, S.B., Heikinheimo, P. & Hough, E. (2006) "The 2.1Å crystal structure of Aerococcus viridans L-lactate oxidase (LOX)." Acta Cryst F. 62, 1185-1190.

Altermark, B., Smalås, A.O., Willassen, N.P. and Helland, R. (2006). "The structure of Vibrio cholerae extracellular endonuclease I reveals the presence of a buried chloride ion." Acta Cryst. D, D62, 1387-91

Sørmo, C.G., Leiros, I., Brembu, T., Winge, P., Kristensen, R., Os, V. and Bones, A.M. (2006). "The crystal structure of Arabidopsis thaliana RAC7/ROP9, a plant specific Rho GTPase."Phytochemistry, 67, 2332-40

Adekoya, O. A, Willassen, N.P. and Sylte, I. (2006)" Molecular insight into pseudolysin inhibition using the MM-PBSA and LIE methods." J. Struct. Biol. 153, 129-44.

Lorentzen, M.S., Moe, E., Jouve, H. and Willassen, N.P. (2006). "Cold adapted features of Vibrio salmonicida catalase. Characterisation and comparison to the mesophilic counterpart from Proteus mirabilis."Extremophiles, 10, 427-440

Brandsdal, B.O., Smalås, A.O. and Åqvist, J. (2006) "Free energy calculations show that acidic P1 variants undergo large pKa shifts upon binding to trypsin. " Proteins.Proteins. 64, 740-748.

Hajjar, E., Korkmaz, B., Gauthier, F., Brandsdal, B.O, Witko-Sarsat, V. and Reuter, N. (2006) "Inspection of the binding sites of proteinase3 and design of a highly specific substrate." J. Med. Chem.49, 1248-1260.

Mekonnen, S.M., Olufsen, M., Smalås, A.O. and Brandsdal, B.O. (2006) "Predicting proteinase specificities from free energy calculations." J. Mol. Gra. Mod. 25, 176-185

Moe, E. Leiros, I., Smalås, A.O. & McSweeney, S. (2006) "The crystal structure of mismatch specific URAClL-DNA glycosylase (MUG) from Deinococcus radiodurans revels a noveal catalytic residue and broad substrate specificity." J. Biol. Chem., 281, 569-577.

Riise, E.K., Lorentzen, M.S., Helland, R. and Willassen, N.P. (2006) "Crystallisation and preliminary X-ray diffraction analysis of a cold adapted catalase from Vibrio salmonicida.”. Acta Cryst. F62, 77-79.

Adekoya, O. A, Helland, R., Willassen, N. P and Sylte, I. (2006) "Comparative sequence and structure analysis reveal features of cold adaptation of an enzyme in the thermolysin family. " Proteins., 62,435-449.

Helland, R., Larsen, A.N., Smalås, A.O. and Willassen, N.P. (2006) The 1.8 Å crystal structure of a Proteinase K like enzyme from a psychrotroph Serratia species." FEBS J., 273, 61-71.

Larsen, A.N., Moe, E., Helland, R., Gjellesvik, D.R. and Willassen, N.P. (2006) "Characterization of a recombinantly expressed proteinase K like enzyme from a psychrotrophic Serratia sp." FEBS J., 273, 47-60.

Almlöf, M., Åqvist, J., Smalås, A.O. & Brandsdal, B.O. (2006) "Probing the effect of point mutations at protein-protein interfaces." Biophys. J., 90, 433-442.

2005
Thorvaldsen, S., Flå, T., and Willassen, N.P. (2005) "Extracting molecular diversity between populations through sequence alignments. Biological and Medical Data Analysis. " In Lecture Notes in Computer Science, Subseries: Lecture Notes in Bioinformatics, Vol. 3745, p317 – 328. Oliveira, J.L.; Maojo, V.; Martin-Sanchez, F.; Sousa Pereira, A. (Eds.)  

Braun, F.N., Paulsen, S., Sear, R.P. and Warren, P.B (2005) "Miscibility gap in the microbial fitness landscape." Phys. Rev. Lett., 94, 178105.

Adekoya, O. A, Willassen, N. P and Sylte, I. (2005) "The protein-protein interaction between SMPI and Thermolysin studied by molecular dynamics and MM/PBSA calculations." J. Biomol. Struct. Dyn., 22, 521-531.

Krowarsch, D., Zakrzewska, M., Smalås, AO., and Otlewski, J. (2005) "Structure-Function Relationships in Serine Protease–Bovine Pancreatic Trypsin Inhibitor Interaction" Protein & Peptide Letter, 12, 403-407.

Leiros, I., Moe, E., Smalås, A.O. and McSweeney, S. (2005) "The crystal structure of Uracil-DNA N-Glycosylase (UNG) from Deinococcus radiodurans." Acta Cryst. D61. 61, 1049-1056.

Zavialov, A.V., Tischebko, V.M., Fooks, L.F., Brandsdal, B.O., Åqvist, J., Zav'yalov, V.P., MacIntyre, S. and Knight S.D. (2005) "Resolving the energy paradox of chaperone/usher-mediated fibre assembly." Biochemical J. 389, 685-694.

Olufsen, M., Smalås, A.O., Moe, E. and Brandsdal, B.O. (2005) "Increased flexibility as a strategy for cold-adaptation: A comparative MD study of cold-and warm-active Uracil DNA glycosylase" J. Biol. Chem.280, 18042-18048.

2004
Leiros, I., McSweeney, S. and Hough, E.(2004) "The reaction mechanism of Phospholipase D from Streptomyces sp. Strain PMF. Snapshots along the reaction pathway reveal a pentacoordinate reaction intermediate and an unexpected final product.." J. Mol. Biol., 339, 805-820.

Czapinska, H., Helland, R., Smalås, A.O. and Otlewski, J. (2004) "Crystal structures of five bovine chymotrypsin complexes with P1 BPTI variants." J. Mol. Biol., 344, 1005-1020.

Moe, E., Leiros, I., Riise, E.K, Olufsen, M., Lanes, O., Smalås, A. O. & Willassen, N. P. (2004). "Optimisation of electrostatic surface potential as strategies for cold adaptation of Uracil DNA glycosylase (UNG) from cod (Gadus morhua)." J. Mol. Biol., 343, 1221-1230.

Leiros, H.-K. S., Brandsdal, B.O. Andersen, O.A., Helland, R.,  Os, V., Otlewski, J., Leiros, I., Willassen, N.P. & Smalås, A.O. (2004) "Trypsin specificity as elucidated by LIE calculations, X-ray structures, and association constant measurements". Protein Science, 13, 1056-1070.

Stenvik, J., Solstad,T.,  Leiros,I.& .Jørgensen, T.Ø. (2004). "Cloning and analysis of the BPI/LBP gene of the Atlantic cod (Gadus morhua)" Journal of Developmental and Comparative Immunology, Vol.28, 307-325

Solem, S.T., Brandsdal, B.O , Smalås, A.O. & Jørgensen, T.Ø. (2004) " The primary structure and specificity determining residues displayed by recombinant salmon antibody domains" Mol. Immunology, Vol. 40:1335-1348.

2003
Andersen OA, Stokka AJ, Flatmark T, Hough E. (2003) "2.0 Å resolution crystal structures of the ternary complexes of human phenylalanine hydroxylase catalytic domain with tetrahydrobiopterin and 3-(2-thienyl)-L-alanine or L-norleucine: substrate specificity and molecular motions related to substrate binding." J Mol Biol. 333(4), 747-757.

Leiros, I., Moe, E., Lanes, O., Smalås, A.O., & Willassen, N.P. (2003). "The crystal structure of uracil-DNA-glycosylase from Atlantic cod (Gadus morhua) reveals cold-adaptation features". Acta Cryst. D59, 1357-1365.

Heikinheimo, P., Helland, R., Leiros, H.K., Leiros,I., Karlsen, S., Evjen, G., Ravelli, R., Schoehn, G., Ruigrok, R., Tollersrud, .O.K., McSweeney, S. and Hough., E. (2003). "The Structure of Bovine Lysosomal alpha-Mannosidase Suggests a Novel Mechanism for Low-pH Activation" J. Mol. Biol. 327(3), 631-644.

Heikinheimo, P., R. Helland, Leiros, H.K., Leiros, I., Karlsen, S., Evjen, G., Ravelli, R., Schoehn, G., Ruigrok, R., Tollersrud, .O.K., McSweeney, S. and Hough., E. (2003). "Structure of the Bovine Lysosomal a-mannosidase, the Enzyme Involved in the Lysosomal Storage Disease a-Mannosidosis" ESRF Highlights 2002: 12-13.

Helland, R., Czapinska, H., Leiros, I., Olufsen, M., Otlewski, J. & Smalås, A.O. (2003) "Structural Consequences of Accomodation of Four Non-cognate Amino Acid Residues in the S1 Pocket of Bovine Trypsin and Chymotrypsin" J. Mol. Biol. 333, 845-861.