BIO-2106 Microscopical imaging Techniques - 10 stp
Nordic applicants: Admission requirements are generell studiekompetanse + REALFA.
Application code: 9336
International applicants: Higher Education Entrance Qualification and certified language requirements in English. A list of the requirements for the Higher Education Entrance Qualification in Norway can be found on the Norwegian Agency for Quality Assurance in Education website - nokut.no
All applicants: The course requires basic knowledge in biology and ecology at university level. Basic knowledge in chemistry and mathematics is an advantage.
Stereo microscope and light microscope are important tools to overcome the limitations of the limited resolving power of the human eye. As they are indispensible for the analysis of prokaryotic and eukaryotic cells, they form an integrative part of biological research.
The course will introduce standard and advanced techniques of light microscopy such as bright field microscopy, phase contrast microscopy and fluorescence microscopy. Strong emphasis will be on the practical part. We will use samples from the fields of microbiology, plant biology and zoology in order to demonstrate the handling of microscopes and familiarize the participating students with themaintenanceof the equipment. Besides the application of various microscopical techniques, we will also treat important related topics such as sample preparation (including fixation, sectioning and staining techniques) and the professional documentation of the results by microphotography. The theoretical part will convey the necessary background knowledge and will cover the composition of light and fluorescence microscopes, magnification and optical resolution, contrast improvement, specific dyes and staining techniques, autofluorescence and secondary fluorescence.
The results of the course will be presented in a small workshop in form of poster presentations and a discussion.
- understand light microscopy as tool for classical and modern biological research
- be comfortable and knowledgeable in working with microscopes
- can select a suitable microscopic technique for a given scientific question
- are familiar with preparation, layout and presentation of a poster concerning their research
The exam consist of a poster presentation at a workshop and a presentaion of relevant literature in a seminar (all on the same day). Grade: passed/failed.
In order to sit an examination, the student must complete and gain approval for any coursework requirements. Coursework requirements: Participation in lab and lectures, approved laboratory report. Implementation of an individual microscopy project
A re-sit exam will be arranged for this course.
Lectures Autumn 2016
First attandance: see schedule
prof. Kirsten Krause
stip. Stian Olsen