DC5
Modelling of microbial influence on NAD metabolisms combining LC-MS/MS methods for NAD and CoA metabolites, global metabolic profiling and metagenomic sequencing
Background: The gut microbiome's potential influence on NAD and CoA metabolism involves the microbial breakdown of dietary compounds, which serve as NAD and CoA precursors, and the presence of bacterial enzymes that impact NAD and CoA biosynthesis and utilization. Additionally, gut-derived factors such as SCFAs, inflammation, and immune interactions may further modulate NAD and CoA metabolism by affecting nutrient absorption and cellular responses. The availability of mice intestinal samples from different sections of the gut allows a precise measurement of where and how metabolites are modified or absorbed.
Objectives: (1) Measure NAD metabolites, global metabolome and the metagenome in samples from mice (2) Elucidate gut-microbiome-NAD and CoA metabolism by identification of specific taxa and metabolic pathways responsible for NAD and CoA precursors synthesis and breakdown (3) Apply integrative multi-omics analysis for modelling the NAD metabolites, metabolic pathways in the gut microbiota.