DC11
SAM and NAD are novel switches in the mTOR network
Background: The PI3K-mTOR kinase network is a metabolic control hub, known to be regulated by phosphorylation and acetylation. We have identified mTOR regulatory proteins harbouring a high proportion of arginines[1] that can be methylated or parylated, and we currently expand the network within the ERC AdG project BEYOND STRESS. In our mTOR interactome we found several arginine and lysine methyl transferases(PRMTs & KMTs), PARPs and 23 ADP-riboslyated proteins, but the impact of these modifications is poorly explored. Objectives:(1) Investigate PRMTs, KMTs and PARPs linking the mTOR kinase network and metabolism (2) Identify the role of hub molecules (NAD, SAM) on mTOR signalling (3) Unravel the interplay of methylation, ADP ribosylation and acetylation on mTOR signalling
[1] Thedieck et al.Cell (2013) https://doi.org/10.1016/j.cell.2013.07.031;Prentzell .. Heiland… Thedieck Cell (2021) https://doi.org/10.1016/j.cell.2020.12.024