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Bilde av Wolfson, Deanna
Bilde av Wolfson, Deanna
Researcher / Group Leader Ultrasound, Microwaves and Optics Department of Physics and Technology deanna.wolfson@uit.no +4777645143 Tromsø You can find me here

Deanna Wolfson

Job description

Application scientist utilizing new techniques in optical microscopy, including both label-free and fluorescence-based techniques. Group leader, biological safety manager, research administration, and anything else that is needed. Check out the "Research and Teaching" or "Publications" tabs for more information!

  • Dhivya Borra Thiyagarajan, Marie Stette, Muhammad Bilal Afzal, Balpreet Singh Ahluwalia, Krishna Agarwal, Roy Ambli Dalmo et al.:
    3D imaging shows nano- and microparticles are internalized by salmon skin and corneal epithelial cells
    Frontiers in Marine Science 2024 ARKIV / DOI
  • Hong Mao, Larissa Dorothea Kruse, Ruomei Li, Ana Oteiza, Eike Christopher Struck, Jasmin Schürstedt et al.:
    Impact of oxidized low-density lipoprotein on rat liver sinusoidal endothelial cell morphology and function.
    npj Gut and Liver 2024 ARKIV / DOI
  • Hong Mao, Karolina Szafranska, Larissa Dorothea Kruse, Christopher Florian Holte, Deanna Wolfson, Balpreet Singh Ahluwalia et al.:
    Effect of caffeine and other xanthines on liver sinusoidal endothelial cell ultrastructure
    Scientific Reports 2023 ARKIV / DOI
  • Florian Ströhl, Deanna Wolfson, Ida Sundvor Opstad, Daniel Henry Hansen, Hong Mao, Balpreet Singh Ahluwalia :
    Label-free superior contrast with c-band ultra-violet extinction microscopy
    Light: Science & Applications (LSA) 2023 ARKIV / DOI
  • Florian Ströhl, Ezra Bruggeman, Christopher Rowlands, Deanna Wolfson, Balpreet Singh Ahluwalia :
    Quantification of the NA dependent change of shape in the image formation of a z-polarised fluorescent molecule using vectorial diffraction simulations
    Microscopy research and technique (Print) 2022 ARKIV / DOI
  • Karolina Szafranska, Tanja Neuman, Zbigniew Baster, Zenon Rajfur, Oskar Szelest, Christopher Florian Holte et al.:
    From fixed-dried to wet-fixed to live-comparative super-resolution microscopy of liver sinusoidal endothelial cell fenestrations
    Nanophotonics 2022 ARKIV / DOI
  • Yakubu Princely Abudu, Birendra Kumar Shrestha, Wenxin Zhang, Anthimi Palara, Hanne Britt Brenne, Kenneth Bowitz Larsen et al.:
    SAMM50 acts with p62 in piecemeal basal- and OXPHOS-induced mitophagy of SAM and MICOS components
    Journal of Cell Biology 2021 ARKIV / DOI
  • Anish Priyadarshi, Firehun Tsige Dullo, Deanna Wolfson, Azeem Ahmad, Nikhil Jayakumar, Vishesh Kumar Dubey et al.:
    A transparent waveguide chip for versatile total internal reflection fluorescence-based microscopy and nanoscopy
    Communications Materials 2021 ARKIV / DOI
  • María Inés Barría, Raymond A. Alvarez, Kenneth Law, Deanna Wolfson, Thomas Huser, Benjamin K. Chen :
    Endocytic motif on a biotin-tagged hiv-1 env modulates the co-transfer of env and gag during cell-to-cell transmission
    Viruses 2021 ARKIV / DOI
  • Liliana Barbieri, Huw Colin-York, Kseniya Korobchevskaya, Di Li, Deanna Wolfson, Narain Karedla et al.:
    Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)
    Nature Communications 2021 ARKIV / DOI
  • Arpita Nepal, Deanna Wolfson, Balpreet Singh Ahluwalia, Ingvill Jensen, Jorunn B Jørgensen, Dimitar Borisov Iliev :
    Intracellular distribution and transcriptional regulation of Atlantic salmon (Salmo salar) Rab5c, 7a and 27a homologs by immune stimuli
    Fish and Shellfish Immunology 2020 ARKIV / DOI
  • Jonathan Hira, Deanna Wolfson, Aaron John Christian Andersen, Tor Haug, Klara Stensvåg :
    Autofluorescence mediated red spherulocyte sorting provides insights into the source of spinochromes in sea urchins
    Scientific Reports 2020 ARKIV / DOI
  • Cristina Ionica Øie, Deanna Wolfson, Tanji Yasunori, Gianina Dumitriu, Karen Kristine Sørensen, Peter Anthony McCourt et al.:
    Liver sinusoidal endothelial cells contribute to the uptake and degradation of entero bacterial viruses.
    Scientific Reports 2020 ARKIV / DOI
  • Rajwinder Singh, Vishesh Dubey, Deanna Wolfson, Azeem Ahmad, Ankit Butola, Ganesh Acharya et al.:
    Quantitative assessment of morphology and sub-cellular changes in macrophages and trophoblasts during inflammation
    Biomedical Optics Express 2020 ARKIV / DOI
  • Ida Sundvor Opstad, Deanna Wolfson, Cristina Ionica Øie, Balpreet Singh Ahluwalia :
    Multi-color imaging of sub-mitochondrial structures in living cells using structured illumination microscopy
    Nanophotonics 2018 ARKIV / DOI
  • Dimitar Borisov Iliev, Guro Strandskog, Arpita Nepal, Augusta Hlin Aspar Sundbø, Randi Olsen, Jorunn B Jørgensen et al.:
    Stimulation of exosome release by extracellular DNA is conserved across multiple cell types
    The FEBS Journal 28. June 2018 ARKIV / DOI
  • Vishesh Dubey, Azeem Ahmad, Rajwinder Singh, Deanna Wolfson, Purusotam Basnet, Ganesh Acharya et al.:
    Multi-modal chip-based fluorescence and quantitative phase microscopy for studying inflammation in macrophages
    Optics Express 2018 ARKIV / DOI
  • Azeem Ahmad, Vishesh Dubey, Vijay Singh, Jean-Claude Tinguely, Cristina Ionica Øie, Deanna Wolfson et al.:
    Quantitative phase microscopy of red blood cells during planar trapping and propulsion
    Lab on a Chip 2018 ARKIV / DOI
  • Robin Diekmann, Deanna Wolfson, Christoph Spahn, Mike Heilemann, Mark Schuttpelz, Thomas Huser :
    Nanoscopy of bacterial cells immobilized by holographic optical tweezers
    Nature Communications 13. December 2016 ARKIV / DOI
  • Chun A. Changou, Deanna Wolfson, Balpreet Singh Ahluwalia, Richard J. Bold, Hsing-Jien Kung, Frank Y.S. Chuang :
    Quantitative analysis of autophagy using advanced 3D fluorescence microscopy
    Journal of Visualized Experiments 2013 DOI
  • Anne Ingeborg Myhr, Deanna Wolfson, Ida Sundvor Opstad, Balpreet Singh Ahluwalia, Dhivya Borra Thiyagarajan, Torill Pauline Bakkelund Blix et al.:
    Nano- and micro-plastics and fish: Novel methods for detection and the role of RRI in such research
    2023
  • Ida Sundvor Opstad, Deanna Wolfson, Balpreet Singh Ahluwalia, Krishna Agarwal, Tore Seternes, Roy Ambli Dalmo :
    Diving into fish skin: understanding cell avalanches and migrating keratocytes
    2022
  • Deanna Wolfson :
    Fishy Business with Biology and Microscopes and People
    2021
  • Ida Sundvor Opstad, Deanna Wolfson :
    Salmon keratocyte DIC microscopy videos
    2021 DATA
  • Deanna Wolfson :
    Practical applications of SIM for biological research
    2020
  • Ida Sundvor Opstad, Daniel Henry Hansen, Ole Kristian Svartaas, Eskil Mossige Kjølstad, Deanna Wolfson, Krishna Agarwal et al.:
    A CHIP-BASED IMAGING SYSTEM FOR FAST-CRAWLING SKIN CELLS OF ATLANTIC SALMON –A COOL SURFACE LAYER INVESTIGATION
    2020
  • Ataur Rahman, Ekaterina Mishchenko, Deanna Wolfson, Hymonti Dey, Johanna U Ericson Sollid, Klara Stensvåg :
    The adhesion and internalization of Staphylococcus aureus into human epithelial cells
    2020
  • Jonathan Hira, Deanna Wolfson, Tor Haug, Klara Stensvåg :
    A simplified and robust method for isolating red spherule cells, and important immune cell of echinoderms
    2020
  • Sebastian Andres Acuña Maldonado, Nikhil Jayakumar, Zicheng Liu, Krishna Agarwal, Deanna Wolfson :
    nanoRIP
    2020
  • Andres Maldonado, Ida Sundvor Opstad, Luis Hernández, Mona Nystad, Milton Aguilera, Merete Storflor et al.:
    Fluorescence Nanoscopy Using Multiple Signal Classification Algorithm (MUSICAL): From Electromagnetics Inverse Scattering Problem to Optics Inverse Source Problem.
    2019
  • Sebastian Andres Acuña Maldonado, Ida Sundvor Opstad, Luis Enrique Villegas Hernandez, Mona Nystad, Milton O Aguilera, Merete Storflor et al.:
    Fluorescence Nanoscopy Using Multiple Signal Classiflcation Algorithm (MUSICAL): From Electromagnetics Inverse Scattering Problem to Optics Inverse Source Problem
    2019
  • Ekaterina Mishchenko, Eric Juskewitz, Hans-Matti Blencke, Deanna Wolfson, Johanna U Ericson Sollid :
    Antibacterial activity of novel marine-derived ”middle-space” molecules: mode of action studies
    2018
  • Deanna Wolfson, Luis Villegas, Ida Sundvor Opstad, Åsa birna Birgisdottir, Cristina Ionica Øie, Balpreet Singh Ahluwalia :
    Structured Illumination Microscopy of Biological Structures
    2018 ARKIV
  • Deanna Wolfson, Cristina Ionica Øie, Tanji Yasunori, Gianina Dumitriu, Peter Anthony McCourt, Karen Kristine Sørensen et al.:
    Uptake and Degradation of Bacteriophages by Liver Sinusoidal Endothelial Cells
    2018 ARKIV
  • Krishna Agarwal, Biswajoy Ghosh, Deanna Wolfson, Cristina Ionica Øie, Jyotirmoy Chatterjee, Balpreet Singh Ahluwalia :
    Computational nanoscopy using Multiple Signal Classification Algorithm (MUSICAL) for fluorescence imaging
    2017
  • Rajwinder Singh, Deanna Wolfson, Vishesh Dubey, Azeem Ahmad, Ganesh Acharya, Dalip Singh Mehta et al.:
    Inflammatory response of macrophages and trophoblasts investigated using structured illumination microscopy and quantitative phase microscopy
    Placenta 2017 ARKIV / DOI
  • Deanna Wolfson, Cristina Ionica Øie, Yasunori Tanji, Karen Kristine Sørensen, Peter Anthony McCourt, Bård Smedsrød et al.:
    Multimodal Microscopy to Study the Fate of Bacteriophages in Liver Sinusoidal Endothelial Cells
    2017
  • Ida Sundvor Opstad, Deanna Wolfson, Cristina Ionica Øie, Balpreet Singh Ahluwalia :
    Three-Color Live Cell Super-Resolution Imaging of Sub-Mitochondrial Regions
    2017
  • Hong Mao, Cristina Ionica Øie, Ruomei Li, Ana Oteiza, Karen Kristine Sørensen, Deanna Wolfson et al.:
    Disruption of rat liver sinusoidal endothelial cell morphology by oxidised low density lipoproteins
    2017
  • Hong Mao, Cristina Ionica Øie, Ruomei Li, Ana Oteiza, Karen Kristine Sørensen, Deanna Wolfson et al.:
    Using super-resolution microscopy to image disruption of liver sinusoidal endothelial cell morphology by oxidized low-density lipoproteins
    2017
  • Deanna Wolfson, Cristina Ionica Øie, Ida Sundvor Opstad, Peter Anthony McCourt, Balpreet Singh Ahluwalia :
    Nanoscale Dynamics of Liver Sinusoidal Endothelial Cells
    2016
  • Robin Diekmann, Øystein Ivar Helle, Cristina Ionica Øie, Ida Sundvor Opstad, Deanna Wolfson, Peter Anthony McCourt et al.:
    Live cell fluctuation-based SR imaging using high-frequency, near-field illumination patterns enabled by planar waveguides
    2016
  • Hong Mao, Cristina Ionica Øie, Ruomei Li, Ana Oteiza, Karen Kristine Sørensen, Deanna Wolfson et al.:
    Disruption of liver sinusoidal endothelial cell morphology by oxidized low–density lipoproteins
    2016
  • Marcel Lahrberg, Mandeep Singh, Deanna Wolfson, Kedar Khare, Balpreet Singh Ahluwalia :
    Enhancements to structured illumination microscopy using blind reconstruction, compressed sensing and evanescent excitation
    2016

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    Research interests

    I work at the interface of biology and microscopy - our group develops new optical microscopy techniques, and I help biologists apply them to their challenging questions in a wide range of biological systems. Topics range from arctic marine biology (e.g. investigating microplastic uptake by salmon skin cells) to antibiotic resistance/susceptibility to organoid models, amongst others. I provide support for many different parts of the research pipeline, such as choosing the right techniques to address a question, experiment design (e.g. helping choose a compatible panel of fluorescent dyes), image acquisition, and image and data analysis. I particularly enjoy matching our toolbox of skills and technologies to otherwise hard-to-solve biological problems, regardless of the type of biology. Give me a good challenge, and that is interesting in itself!

    Other key areas for me are improving and developing systems to facilitate research and research administration. Particularly, developing research infrastructure for biology (including biosafety as well as lab facilities) in a physical sciences faculty has been an interesting challenge, as well as scaling up support systems to accomadate the needs of a group that has tripled in size over the last decade.

    Teaching

    I provide training on the use of commercial fluorescent and label-free microscopes, basic image analysis using ImageJ, basic cell culture and sterile handling, and a range of microscopy-related topics on request. Intro to Microscopy (for biologists and computer scientists) and Intro to Cell Culture (for physicists and computer scientists) were offered as special curricula in 2022. Biosafety (for BSL2 and genetically modified material) training is provided on a regular basis to group members and collaborators as needed. I occasionally conduct workshops and provide training related to interdisciplinary science and scientific communication.

    I have co-supervised master's and PhD students both formally and informally within physics and in fishery science; however, the vast majority of my teaching and outreach activities are conducted outside formal teaching structures and on an as-needed basis.

    ORCID: https://orcid.org/0000-0001-6059-2472

    Member of research group

    Ultrasound, Microwaves and Optics   Norsk innhold Optical Nanoscopy   English content
    Teknologibygget Tromsø 3.049


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