Course code: BIO-8104

Applied molecular genetics

Campus
Semester / Year Autumn 2012
Credits 20

About the course

The aim with this course is to give a thorough introduction to molecular genetic methods used in modern biological research. All lectures will be closely connected to the practical problems to be solved in the laboratory. Written laboratory reports will be subjects for plenary discussions as well as literature seminars and a mandatory report. The course will give a basis for advanced studies in different areas of microbiology and will therefore contain problems concerning medicine, ecology and food-microbiology.

Four practical problems are planned within the following topics: Taxonomy, genetic diversity, identification and characterisation, molecular microbal ecology and horizontal gene transfer.

The following methods will be used: rep-PCR-fingerprinting, 16S-RFLP, fluorescence in situ hybridisation (FISH), microarray, real-time PCR, Pulsed field gel electrophoresis (PFGE), conjugation and transformation. The teaching will also address bioinformatic tools for phylogeny and microarray technology.

Prerequisites

Credit reduction

If you pass the examination in this course, you will get an reduction in credits (as stated below), if you previously have passed the following courses:

  • BIO-3134 Applied Molecular Genetics 20 stp

Teaching methods

Lectures 10 hours, field and laboratory exercises 60 hours, seminars and project work 40 hours.

Language of instruction and examination

The language of instruction is English and all of the syllabus material is in English. Examination questions will be given in English, but may be answered either in English or a Scandinavian language.

Assessment

Laboratory report, seminars based on submitted written material from group work, graded project essay, 4 h written exam with grades. Letter grades, A-F.

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Responsible unit: Department of Arctic and Marine Biology