Md Jalal Uddin
Job description
My current research employs a chemoproteomic approach known as activity-based protein profiling (ABPP) to identify and validate druggable enzymes relevant to bacterial survival and pathogenesis. Additionally, I use protein biochemistry, various in vitro assays, human-derived gut organoid models, Galleria infection model, and microscopy to explore the functional roles of these enzymes in diverse biological contexts.
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Publications outside Cristin
Md Jalal Uddin , Marco C. Viveen, Janetta Top, Mona Johannessen, Marcel R. de Zoete , Rob JL Willems and Christian S. Lentz. 2023. Chemoproteomic Identification of Klebsiella pneumoniae Serine Hydrolases with Potential Roles in Initial Fitness in Human-Derived Intestinal Organoids. EMBO | EMBL Symposium: Organoids: modeling organ development and disease in 3D culture 2023, EMBL Heidelberg, Germany.
Md Jalal Uddin , Marco C. Viveen, Janetta Top, Mona Johannessen, Marcel R. de Zoete , Rob JL Willems and Christian S. Lentz. 2023. Activity-Based Protein Profiling identifies Klebsiella pneumoniae
Serine Hydrolases With Potential Roles in Host-Pathogen Interactions. FEMS Microbiology Conference, Hamburg, Germany.
Md Jalal Uddin , Jirapat Dawan, Gibeom Jeon, Jongwon Lee, Tao Yu, Xinlong He, Juhee Ahn. The Role of Bacterial Membrane Vesicles in the Dissemination of Antibiotic Resistance and as Promising Carriers for Therapeutic Agent Delivery. Journal of microorganisms, MDPI. DOI: 10.3390/microorganisms8050670. review article
Md. Jalal Uddin , Juhee Ahn. Associations between antibiotic resistance and bacteriophage resistance phenotypes in laboratory and clinical strains of Salmonella enterica subsp. enterica serovar Typhimurium. Journal of Microbial Pathogenesis. DOI: 10.1016/j.micpath.2020.104159.
Shuai Wei, Ramachandran Chelliah, Momna Rubab, Deog-Hwan Oh, Md Jalal Uddin , Juhee Ahn. Bacteriophages as Potential Tools for Detection and Control of Salmonella spp . in Food Systems. Journal of microorganisms, MDPI. DOI: 10.3390/microorganisms7110570. review article
Md Jalal Uddin , Beomseok Kim, Jirapat Dawan, Tian Ding, Jin-Chul Kim, Juhee Ahn. Assessment of antibiotic resistance in bacteriophage-insensitive Klebsiella pneumoniae. Journal of Microbial Pathogenesis. DOI: 10.1016/j.micpath.2019.103625
Md Jalal Uddin , Choong Je Ma, Jin-Chul Kim, Juhee Ahn. 2019. Proteomics-based discrimination of differentially expressed proteins in antibiotic-sensitive and antibiotic-resistant Salmonella Typhimurium, Klebsiella pneumoniae , and Staphylococcus aureus . Archives of Microbiology. DOI: 10.1007/s00203-019-01693-1.
Md. Jalal Uddin , Juhee Ahn. 2017. Associations between resistance phenotype and gene expression in response to serial exposure to oxacillin and ciprofloxacin in Staphylococcus aureus . Letters in Applied Microbiology. DOI: 65: 462-468. doi: 10.1111/lam.12808
Islam MJ, Uddin MJ , Hossain MA, Henry R, Begum MK, et al. (2022) Exogenous putrescine attenuates the negative impact of drought stress by modulating physio-biochemical traits and gene expression in sugar beet (Beta vulgaris L.). PLoS ONE 17 (1): e0262099. https://doi.org/10.1371/journal.pone.0262099
Jirapat Dawan, Md Jalal Uddin , Juhee Ahn, Development of de novo resistance in Salmonella Typhimurium treated with antibiotic combinations, FEMS Microbiology Letters, Volume 366, Issue 10, May 2019, fnz127, https://doi.org/10.1093/femsle/ fnz127
Research interests
Research interests
My previous work
I worked on the 'Phage Therapy Project,' where we utilized phages, phage-antibiotic combinations, and phage products to develop more effective phage-based therapies for controlling multidrug-resistant pathogens such as Salmonella Typhimurium, Klebsiella pneumoniae , and Staphylococcus aureus . And, wrote my master's thesis entitled 'Characterization of Virulent Bacteriophages to Control Multidrug-Resistant Salmonella Typhimurium.
Current work
My current research focuses on employing a chemoproteomic approach called activity-based protein profiling (ABPP) and microbiological techniques to study bacterial pathogens. We use functionalized covalent enzyme inhibitors, tagged with biotin or click-chemistry handles, to enrich and identify target enzymes relevant to bacterial survival and pathogenesis, and for comparing active proteomes under various biological conditions. For functional validation of these druggable enzymes, we utilize protein biochemistry, bacterial mutant/reporter strains, and chemical probes in different in vitro assays, human-derived gut organoid models, the Galleria infection model, and in vivo animal assays .
- Chemical biology
- Organoids
- Phage therapy
- OMVs
Teaching
- Biomedicine master course , MBI3012- Chemical Biology part
- Biomedicine bachelor course MBI1000- Enzymes kinetics, biochemistry
