Johnsen, Pål Jarle
Christopher Frøhlich,
Vidar Sørum,
Nobuhiko Tokuriki,
Pål Jarle Johnsen,
Ørjan Samuelsen
:
Evolution of β-lactamase-mediated cefiderocol resistance
Vidar Sørum,
Emma Lu Øynes,
Anna Sollied Møller,
Klaus Harms,
Ørjan Samuelsen,
Nicole L. Podnecky
et al.:
Evolutionary Instability of Collateral Susceptibility Networks in Ciprofloxacin-Resistant Clinical Escherichia coli Strains
Macaulay Winter,
Angus Buckling,
Klaus Harms,
Pål Jarle Johnsen,
Michiel Vos
:
Antimicrobial resistance acquisition via natural transformation: context is everything
Jonina Gudmundsdottir,
Elizabeth G. Aarag Fredheim,
Catharina I.M. Koumans,
Joachim Hegstad,
Po-Cheng Tang,
Dan I. Andersson
et al.:
The chemotherapeutic drug methotrexate selects for antibiotic resistance
Rebecca Ashley Gladstone,
Alan McNally,
Anna Kaarina Pöntinen,
Gerry Tonkin-Hill,
John A. Lees,
Kusti Onni Skytén
et al.:
Emergence and dissemination of antimicrobial resistance in Escherichia coli causing bloodstream infections in Norway in 2002–17: a nationwide, longitudinal, microbial population genomic study
Pål Jarle Johnsen,
João Alves Gama,
Klaus Harms
:
Bacterial evolution on demand
Julia Kloos,
João A Gama,
Joachim Hegstad,
Ørjan Samuelsen,
Pål J Johnsen
:
Piggybacking on Niche Adaptation Improves the Maintenance of Multidrug-Resistance Plasmids
Sergio Arredondo Alonso,
Anna Kaarina Pöntinen,
Francois Pierre Alexandre Cleon,
Rebecca Ashley Gladstone,
Anita C. Schürch,
Pål Jarle Johnsen
et al.:
A high-throughput multiplexing and selection strategy to complete bacterial genomes
Christopher Frøhlich,
João Alves Gama,
Klaus Harms,
Viivi H. A. Hirvonen,
Bjarte Aarmo Lund,
Marc W. van der Kamp
et al.:
Cryptic β-Lactamase Evolution Is Driven by Low β-Lactam Concentrations
Joao Pedro Alves Gama,
Julia Maria Kloos,
Pål Jarle Johnsen,
Ørjan Samuelsen
:
Host dependent maintenance of a blaNDM-1-encoding plasmid in clinical Escherichia coli isolates
Julia Maria Kloos,
Joao Pedro Alves Gama,
Pål Jarle Johnsen,
Ørjan Samuelsen
:
Host dependent maintenance of a blaNDM-1-encoding plasmid in clinical Escherichia coli isolates
Scientific Reports 2020 DOI
Julia Maria Kloos,
Pål Jarle Johnsen,
Klaus Harms
:
Tn1 transposition in the course of natural transformation enables horizontal antibiotic resistance spread in Acinetobacter baylyi
Christopher Frøhlich,
Vidar Sørum,
Ane Molden Thomassen,
Pål Jarle Johnsen,
Hanna-Kirsti S. Leiros,
Ørjan Samuelsen
:
OXA-48-Mediated Ceftazidime-Avibactam Resistance Is Associated with Evolutionary Trade-Offs
Nicole L. Podnecky,
Elizabeth Aarag,
Julia Maria Kloos,
Vidar Sørum,
Raul Primicerio,
Adam Paul Roberts
et al.:
Conserved collateral antibiotic susceptibility networks in diverse clinical strains of Escherichia coli.
Nils Fredrik Hülter,
Vidar Sørum,
Kristina Borch-Pedersen,
Mikkel Meyn Liljegren,
Ane Utnes,
Raul Primicerio
et al.:
Costs and benefits of natural transformation in Acinetobacter baylyi
Maria Chiara Di Luca,
Vidar Sørum,
Irina Starikova,
Julia Maria Kloos,
Nils Fredrik Hülter,
Mohammed Umaer Naseer
et al.:
Low biological cost of carbapenemase-encoding plasmids following transfer from Klebsiella pneumoniae to Escherichia coli
Journal of Antimicrobial Chemotherapy 2016 DOI
Klaus Harms,
Asbjørn Lunnan,
Nils Hulter,
Tobias Mourier,
Lasse Vinner,
Cheryl P Andam
et al.:
Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms
Jan Engelstädter,
Klaus Harms,
Pål Jarle Johnsen
:
The evolutionary dynamics of integrons in changing environments
The ISME Journal 2016 DOI
Amy Mcleman,
Pawel Sierocinski,
E Hesse,
A Buckling,
G Perron,
Nils Fredrik Hülter
et al.:
No effect of natural transformation on the evolution of resistance to bacteriophages in the Acinetobacter baylyi model system.
Ole Herman Ambur,
Jan Engelstädter,
Pål Jarle Johnsen,
Eric L. Miller,
Daniel E. Rozen
:
Steady at the wheel: Conservative sex and the benefits of bacterial transformation
Luigimaria Borruso,
Klaus Harms,
Pål Jarle Johnsen,
Kaare Magne Nielsen,
Lorenzo Brusetti
:
Distribution of class 1 integrons in a highly impacted catchment
Ane Live Gunnes Utnes,
Vidar Sørum,
Nils Fredrik Hülter,
Raul Primicerio,
Joachim Hegstad,
Julia Maria Kloos
et al.:
Growth phase-specific evolutionary benefits of natural transformation in Acinetobacter baylyi
The ISME Journal 2015 DOI
Iren Høyland Löhr,
Nils Fredrik Hülter,
Eva Bernhoff,
Pål Jarle Johnsen,
Arnfinn Sundsfjord,
Mohammed Umaer Naseer
:
Persistence of a pKPN3-like CTX-M-15-encoding IncFIIK plasmid in a Klebsiella pneumonia ST17 host during two years of intestinal colonization
Bruce R: Levin,
Fernando Baquero,
Pål Jarle Johnsen
:
A model-guided analysis and perspective on the evolution and epidemiology of antibiotic resistance and its future
Current Opinion in Microbiology 2014 DOI
Shweta Fulsundar,
Klaus Harms,
Gøril Eide Flaten,
Pål Jarle Johnsen,
Balu Ananda Chopade,
Kaare Magne Nielsen
:
Gene transfer potential of outer membrane vesicles of Acinetobacter baylyi and effects of stress on vesiculation
Søren Overballe-Petersen,
Klaus Harms,
Ludovic A.A. Orlando,
J. Victor Moreno Mayar,
Simon Rasmussen,
Tais W. Dahl
et al.:
Bacterial natural transformation by highly fragmented and damaged DNA
Proceedings of the National Academy of Sciences of the United States of America 2013 DOI
Irina Starikova,
Mohammed Al-Haroni,
Guido Werner,
Adam P. Roberts,
Vidar Sørum,
Kåre M. Nielsen
et al.:
Fitness costs of various mobile genetic elements in Enterococcus faecium and Enterococcus faecalis
Sara Domingues,
Klaus Harms,
W. Florian Fricke,
Pål Jarle Johnsen,
Gabriela J. da Silva,
Kåre M. Nielsen
:
Natural Transformation Facilitates Transfer of Transposons, Integrons and Gene Cassettes between Bacterial Species
Irina Starikova,
Klaus Harms,
Pål Haugen,
Tracy Munthali Lunde,
Raul Primicerio,
Ørjan Samuelsen
et al.:
A Trade-off between the Fitness Cost of Functional Integrases and Long-trem stability of Integrons
Kåre M. Nielsen,
Jessica Louise Ray,
Pål Jarle Johnsen
:
Horizontal gene transfer: the uptake of extracellular DNA in bacteria
Elsevier 2012
Julia Hurler,
Ole Alexander Berg,
Merete L. Skar,
Anne Hilde Conradi,
Pål Jarle Johnsen,
Natasa Skalko-Basnet
:
Improved burns therapy: Liposomes-in-hydrogel delivery system for mupirocin
Journal of Pharmaceutical Sciences 2012 DOI
Julia Maria Kloos,
Lea Jänisch,
Anna Kaarina Pöntinen,
Ørjan Samuelsen,
Pål Jarle Johnsen,
Jukka Corander
et al.:
Different metabolic proficiency among dominant ExPEC lineages
2022
Christopher Frøhlich,
Adrian H. Bunzel,
Marc W. Van Der Kamp,
Adrian J. Mulholland,
Hanna-Kirsti Schrøder Leiros,
Pål Jarle Johnsen
et al.:
Positive epistasis drives the molecular evolution of the carbapenemase OXA-48
2022
João Alves Gama,
Julia Maria Kloos,
Joachim Hegstad,
Ørjan Samuelsen,
Pål Jarle Johnsen
:
Plasmid cost alleviation as a consequence of niche-adaptation
2021
Julia Maria Kloos,
João Alves Gama,
Joachim Hegstad,
Ørjan Samuelsen,
Pål Jarle Johnsen
:
Plasmid Cost Alleviation By Hitchhiking On Niche-adaptation
2021
Julia Maria Kloos,
Joao Pedro Alves Gama,
Joachim Hegstad,
Ørjan Samuelsen,
Pål Jarle Johnsen
:
E. coli Niche Adaptation Improves Plasmid Maintenance
2021
Sybil Akua Okyerewa Obuobi,
Kjersti Julin,
Elizabeth G. Aarag Fredheim,
Pål Jarle Johnsen,
Mona Johannessen,
Natasa Skalko-Basnet
:
Liposomal delivery of antibiotic loaded DNA nanogels against intracellular infections
2020
Vidar Sørum,
Anna Sollied Møller,
Pål Jarle Johnsen,
Ørjan Samuelsen,
Sören Abel,
Nicole L. Podnecky
:
Evolutionary stability of collateral effects in ciprofloxacin resistant clinical Escherichia coli strains
2019
Vidar Sørum,
Anna Sollied Møller,
Sören Abel,
Ørjan Samuelsen,
Nicole L. Podnecky,
Pål Jarle Johnsen
:
Evolutionary stability of collateral effects in ciprofloxacin resistant clinical Escherichia coli strains
2019
Vidar Sørum,
Emma Lu Øynes,
Anna Møller,
Nicole L. Podnecky,
Sören Abel,
Ørjan Samuelsen
et al.:
Evolutionary stability of collateral susceptibility networks in clinical Escherichia coli strains
2019
Elizabeth G. Aarag Fredheim,
Nicole Podnecky,
Angie Carola Alarcon Rios,
Jessica Nguyen Tran,
Chantal Verstappen,
Klaus Harms
et al.:
Gentamicin collateral sensitivity of ciprofloxacin resistant Escherichia coli is opposed by the rapid development of gentamicin resistance
2019
Christopher Frøhlich,
Hanna-Kirsti S. Leiros,
Pål Jarle Johnsen,
Ørjan Samuelsen
:
Sub-inhibitory concentrations of ceftazidime drive evolution of the OXA-48 carbapenemase
2019
Christopher Frøhlich,
Pål Jarle Johnsen,
Hanna-Kirsti Schrøder Leiros,
Ørjan Samuelsen
:
Evolution of OXA-48 under sub-MIC conditions
2019
Christopher Frøhlich,
Vidar Sørum,
Hanna-Kirsti S. Leiros,
Pål Jarle Johnsen,
Ørjan Samuelsen
:
Development of OXA-48 mediated ceftazidme-avibactam resistance is associated with an evolutionary trade-off
2018
Julia Maria Kloos,
Joao Pedro Alves Gama,
Ørjan Samuelsen,
Pål Jarle Johnsen
:
Klebsiella pneumoniae MDR-encoding plasmids in clinical isolates of uropathogenic Escherichia coli: plasmid-host co-evolution and its effect on fitness cost of plasmid-mediated resistance
2018
Julia Maria Kloos,
Joao Pedro Alves Gama,
Ørjan Samuelsen,
Pål Jarle Johnsen
:
Plasmid-host co-evolution of Klebsiella pneumoniae MDR-encoding plasmids in clinical isolates of uropathogenic Escherichia coli: effect on fitness cost of plasmid-mediated resistance
2018
Julia Maria Kloos,
Kaare Magne Nielsen,
Pål Jarle Johnsen,
Klaus Harms
:
Natural transformation of Acinetobacter baylyi by transposons
2017
Julia Maria Kloos,
Kaare Magne Nielsen,
Pål Jarle Johnsen,
Klaus Harms
:
Transposition during natural transformation facilitates spread of antibiotic resistance determinants in Acinetobacter baylyi
2016
Klaus Harms,
Irina Starikova,
Pål Jarle Johnsen
:
Costly Class-1 integrons and the domestication of the the functional integrase
Mobile Genetic Elements 2013 DOI
Pål Jarle Johnsen
:
Is it more to bacterial sex than exploring the fitness landscape of other organisms?
2012
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Research interests
I'm head of the Microbial Pharmacology and Population Biology Research Group (MicroPop) and generally interested in bacterial microevolution and focusing on two main topics.
1. Evolution, selection, and spread of antibiotic resistance: It is clear that the frequency of antimicrobial drug resistance in the community is associated with the total level of antimicrobial consumption. Not so clear is the association between reduced consumption levels and subsequent reduction in the frequency of resistance. We focus on different processes that may counteract the reversal of antimicrobial resistance in drug free environments such as the biological cost of resistance, horizontal gene transfer, and genetic stability of resistance determinants. We are currently expanding our activities to include approaches for optimized antimicrobial treatment informed by principles of evolutionary, and population biology.
2. Mechanistic and evolutionary consequences of bacterial recombination: Horizontal gene transfer (HGT) in bacteria plays a major role in adaptive evolution exemplified by the evolution, spread and selection for antibiotic resistance determinants. For two out of three described mechanisms of HGT, conjugation and transduction, gene recruitment through recombination is likely a coincidental byproduct of plasmids’ and phages’ need for continuous transmission to new hosts. The third mechanism of HGT, natural transformation, is a complex mechanism for DNA uptake that requires the concerted action of many chromosomal genes. On first consideration it appears evident that this mechanism for HGT has evolved to “sample the available sequence space” in order to increase the adaptation rates of transformable strains. However, other hypotheses exist for both the evolution and the maintenance of natural transformation in bacteria. We are currently testing a number of these hypotheses both experimentally and theoretically. We are also interested in the mechanistic basis of bacterial recombination and it affects genetic diversity in microbes.
CV AND CURRENT POSITION
2000-2004. Ph.D. Student Supervisors Prof. Arnfinn Sundsfjord, Dr. Med. Gunnar Skov Simonsen
2004-2005 . Post Doctoral Fellowship. Department of Pharmacology. Prof. Kaare M. Nielsen Lab, Institute of Pharmacy, University of Tromsø, Norway.
2006-2008: Personal postdoc grant The Norwegian Research Council (NFR), including:
2007-2008 Visiting Post Doc. Bruce Levin Lab. Emory University, Atlanta, USA
2008- Associate Professor of Microbiology. Research group for Microbiology, pharmaco- and molecular epidemiology, Department of Pharmacy, University of Tromsø. Group leader: Prof. Kaare M. Nielsen.
2014- Associate Professor, Group Leader Microbial Pharmacology and Population Biology Research Group
2015: Full Professor, Group Leader Microbial Pharmacology and Population Biology Research Group
SELECTED PUBLICATIONS
- Harms K., Lunnan A.,Hülter N., Mourier T., Vinner L., Andam C.P., Marttinen P., Fridholm H., Hansen A.J., Hanage W.P., Nielsen K.M., Willerslev E., Johnsen P.J. Substitutions of short heterologous DNA segments of intra- or extragenomic origins produce clustered genomic polymorphisms. Proc Natl Acad Sci U S A, In press
- Ambur OH., Engelstädter J., Johnsen PJ., Miller EL., Rozen DE. Steady at the wheel: conservative sex and the benefits of natural transformation. Philos Trans R Soc Lond B Biol Sci. 2016 Oct 19;371(1706). pii: 20150528.
- Di Luca MC., Sørum V., Starikova I., Klos J., Hülter N., Naseer U., Johnsen PJ., Samuelsen Ø. Low biological cost of carbapenemase producing plasmids following transfer from Klebsiella pneumonia to Escherichia coli. J Antimicrob Chemother. 2016 Sep 2. pii: dkw350
- Engelstädter J., Harms K., Johnsen PJ. The evolutionary dynamics of integrons in changing environments ISME J, 2016, Jun;10(6):1296-307.
- Utnes A., Sorum V., Hulter N., Primicerio R., Kloos J., Nielsen K.M., Johnsen PJ. Growth phase specific evolutionary benefits of natural transformation in Acinetobacter baylyi. ISME J, 2015;Oct;9(10):2221-31.
- Levin B. R., Baquero F., Johnsen PJ. A Model-Guided Analysis and Perspective on the Evolution and Epidemiology of Antibiotic Resistance and its Future. Invited Current Opin Microbiol. 2014: Jun;19:83-9
- Overballe-Petersen S., Harms K*., Orlando L. A., Mayar J. V., Rasmussen S., Dahl T. W., Rosing M. T., Poole A. M., Sicheritz-Ponten T., Brunak S., Inselmann S., de Vries J., Wackernagel W., Pybus O. G., Nielsen R., Johnsen P. J., Nielsen K. M., and Willerslev E. Bacterial natural transformation by highly fragmented and damaged DNA. Proc Natl Acad Sci U S A. 2013: 110(49):19860-5. doi: 10.1073/pnas.1315278110. Epub 2013 Nov 18.
- Starikova I., Al-Haroni M., Werner G., Roberts A. P., Sørum V., Nielsen K.M., Johnsen PJ. Fitness costs of mobile genetic elements in Enterococcus faecium and Enterococcus faecalis.J Antimicrob Chemother. 2013 Dec;68(12):2755-65. Epub 2013 Jul 5.
- Harms K., Starikova I., Johnsen PJ. Costly class 1 integrons and the domesticatioon of the functional integrase. Mob Genet Elements. 2013 Mar 1;3(2):e24774
- StarikovaI., Harms K., Lunde T. M., Haugen P., Primicerio R., Samuelsen Ø., Nielsen K. M, Johnsen PJ. 2012. A trade-off between the fitness cost of functional integrases and long-term stability of integrons. PloS Pathog.8(11):e1003043.
- Domingues S., Harms K., Fricke F. W., Johnsen P. J., da Silva G., and K. M. Nielsen. 2012. Natural Transformation Facilitates Transfer of Transposons, Integrons and Gene Cassettes between Bacterial Species. PloS Pathog. 8 (8): e1002837
- Johnsen PJ., Townsend J. P., Bøhn T., Simonsen G. S., Sundsfjord A., and Nielsen K. M. 2011. Retrospective evidence for a biolog10ical cost of vancomycin resistance in Enterococcus faecium. J. Antimicrobial Agents and Chemother. 66(3):608-10
- Johnsen PJ., and Levin B. R. 2010. Adjusting to alien genes. Mol Microbiol. 75: 1061-63 Invited commentary
- Ray, J. L., Harms K., O. G. Wikmark, Starikova I., Johnsen PJ Nielsen KM. 2009. Sexual isolation in Acinetobacter baylyi is locus-specific and varies 10 000-fold over the genome. Genetics. 182: 1165-81.
- Johnsen PJ., Townsend J., Bohn T., Simonsen GS., Sundsfjord A., Nielsen KM. 2009. Factors affecting the reversal of antimicrobial resistance. Lancet Infect. Dis. 9: 357-364.
- Johnsen PJ., Dubnau D., Levin BR. Episodic Selection and the Maintenance of Competence and Transformation in Bacillus subtilis. 2009. Genetics. 181: 1521-1533. Chosen as Editorial highlight for April 2009
Member of research group
FARM F4. 218