Arctic Bioanalytical Platform

Our bioanalytical platform consists of a wide range of instrumentation and techniques which allow us to identify, isolate, and characterise novel bioactive compounds.

With a focus on metabolites from marine organisms, among other objectives, our group works towards the discovery of new antibiotic compounds. The isolation of compounds is achieved with a combination of semi-preparative HPLC-DAD fractionation, and metabolite identification via ultra-high performance liquid chromatography coupled to a diode array detector and high resolution mass spectrometer (UHPLC-DAD-QTOF-MS).

Our large range of different analytical and preparative columns allow us to implement various modes of separation, including reversed phase, normal phase, HILIC, and size exclusion, resulting in the ability to investigate metabolites with a wide range chemical characteristics.The biological activity of these marine metabolites and extracts are determined by our various bioassays. These bioassays are used to monitor HPLC-DAD fractionation, access activity of purified compounds, and to investigate the mode-of-action of the compounds.

For more information about our platform, the services we can provide, and collaborations feel free to contact one of our group members:

Prof. Klara Stensvåg (klara.stensvag@uit.no): Natural products, biological analysis, function of molecules, genes
Prof. Tor Haug (tor.haug@uit.no): Natural products, characterization, isolation
Assoc. Prof. Hans-Matti Blencke (hans-matti.blencke@uit.no): Biological analysis
Assoc. Prof. Gabriel De Freitas Almeida (gabriel.d.almeida@uit.no ): Virus; bacteriophages, giant virus
Senior engineer, Dr. Ida Kristine Østnes Hansen (ida.k.hansen@uit.no ): HPLC, LC-QTOF-MS, MALDI-TOF

INSTRUMENTATION
  • HPLC-DAD
  • UHPLC-DAD-QTOF-MS
  • MALDI-TOF MS
  • Fluorescence microscopy
  • Flow cytometry
  • Multi-plate reader
EXPERTISE
  • Purification of natural products
  • Bioassays testing (anti-bacterial, anti-fungal, biofilm inhibition)
  • De novo sequencing of peptides
  • Mass spectrometry based metabolomics and database development
  • UHPLC-DAD and MS quantification
  • Basic virus work, bacteriaphages, giant virus
LINKS

Attachments:
LCMS Sample Submission