NMR is a powerful technique for fragment/ligand screening. It's strength lies in its ability to detect very weak binding (mM or stronger) and most methods do not require any isotop labelling or immobilization, but is performed directly on the native enzyme in aqueous solution. Cocktails of 10-30 compounds can be applied simultaneously and directly reveal which of the molecules in the cocktail that binds to the protein.

Ligand detected methods

Protein detected methods