DYRK2, analogs of DYRKs
The Dual-specificity tYrosine phosphorylation-Regulated Kinases (DYRKs) are called “dualspecificity” kinases because of their ability to become active by autophosphorylation of a tyrosine residue in the activation segment during translation. DYRKs are an evolutionary conserved family whose members are found in different branches of life: Yeast, nematodes, drosophila, vertebrates, etc... . Human homologs of DYRKs belong to the CMGC group which also includes the cyclin-depended kinases (CDKs), mitogen-activated protein kinases (MAPKs), glycogen-synthase kinase-3 (GSK3) and CDK-like kinases. The human DYRK family includes five members: DYRK1A, DYRK1B, DYRK2, DYRK3 and DYRK4.
The project includes:
Expression and purification of DYRK2 and/or DYRKs from other species
Cocrystallization of DYRK2 and/or DYRKs from other species
Inhibitor screening, activity assays