Projects

The importance of hypoxia/angiogenesis, immune cells and epithelial-mesenchymal transformation in tumor and stromal cells of lung cancer    

On the basis of biological material in TMA from 335 operated non-small cell lung cancers we are studying the prognostic significance of immune cells in addition to a number of molecular "families" within hypoxia/angiogenesis and EMT.

This has so far resulted in a number of exciting results published in reputable international journals and four doctoral degrees. We will continue to immerse ourselves in these fields with emphasis on markers with clinical relevance and the strong interactions between the different families and an exciting complex interaction between tumor cells and stromal cells.

Contacts:

Profesor/MD Tom Dønnem, tom.donnem@uit.no, 776 45427

Research fellow/MD Sigve Andersen, sigve.andersen@uit.no, 776 46447

Professor Roy M. Bremnes, roy.bremnes@uit.no, 776 26807


Identification of prognostic markers of non-GIST soft tissue sarcomas
Soft tissue sarcoma (STS) is an aggressive tumor group that constitutes less than 1% of all malignant tumors. On the basis of biological material in TMAs from 249 STS patients we are studying the prognostic significance of immune cells in addition to a number of molecular "families" within hypoxia/angiogenesis, hormone receptors and EMT. This has so far resulted in exciting results published in reputable international journals.

Contacts:

Research fellow/MD Sveinung Sørbye, sveinung.sorbye@unn.no, 776 27223

Professor Roy M. Bremnes, roy.bremnes@uit.no, 776 26807

Professor Lill-Tove Busund, lill-tove.busund@unn.no, 776 27221


Prognostic significance of immune cells and families of molecular markers of prostate cancer.
The risk of developing prostate cancer increases with increasing age. Number of newly detected cases has increased sharply in recent years, and our mortality rates are among the highest in the world.

On the basis of tumor tissue (TMA) we are studying the prognostic significance of immune cells and other stromal cells for cancer development. Besides we are studying a wide range of molecular markers in hypoxia/angiogenesis and EMT in both tumor and stromal cells.

Contacts:

Assist prof/MD: Elin Richardsen, elin.richardsen@unn.no, 776 27208

Postdoc/MD Sigve Andersen, sigve.andersen@uit.no, 776 46447

Professor Lill-Tove Busund, lill-tove.busund@unn.no, 776 27221


Basic and clinical radiation biology by studying the responses by radiation therapy on stromal and malignant cells from resected lung tumors.
The main efforts of our research are currently directed towards the impact of ionizing radiation on the different cell types encountered in solid malignancies, aiming to identify molecular mechanisms for proliferation and migration.

All experiments in this project are performed with freshly resected human tumor cells obtained from resected tumor samples. The experimental strategy includes a wide range of molecular and cell biological techniques including high throughput gene and protein profiling, as the specific gene and protein determination, cell and tissue imaging and functional cell assays.

Contacts:

Postdoc Ingvild Pettersen, ingvild.pettersen@uit.no, 776 46835

Professor Inigo Martinez, inigo.martinez@uit.no, 776 45449/44686

Professor Roy Bremnes, roy.bremnes@unn.no, 776 26807


MicroRNA - small but important regulators of gene expression
MicroRNA (miRNA) are short single-threaded, non-coding RNA fragments that regulate gene expression posttranscriptional by binding to mRNA and prevent translation. Today more than thousand human miRNA are registered, and many of these are deregulated in tumor cells versus normal cells.

We use PCR and/or in situ hybridization on tissue microarrays (ISH) to determine the expression of different miRNA and their prognostic and diagnostic role.

Contacts:

Assistant Professor Thomas Berg, thomas.berg@unn.no, 776 45482

Professor/MD Tom Dønnem, tom.donnem@uit.no, 776 45427

Professor Lill-Tove Busund, lill.tove.busund@unn.no, 776 27221


Cardiovascular disease in men who are cured of testicular cancer
We have previously in several large, national studies shown that chemotherapy and/or radiotherapy in the treatment of testicular cancer increases the risk of cardiovascular disease.

We want to study this further by examining whether the essential chemotherapy agent, cisplatin, is measurable in blood many years after treatment and whether cisplatin in blood is associated with risk of cardiovascular and pulmonary side effects. We also plan to investigate how many patients die from heart disease after successful testicular cancer treatment.

Contacts:

Research fellow/MD Line Veronica Hjelle, line.v.hjelle@uit.no, 776 45427 

Assist. Prof. /MD Hege S. Haugnes, hege.sagstuen.haugnes@unn.no, 776 54342

Professor Roy Bremnes, roy.bremnes@unn.no, 776 26807


Effects of PAX6 in cell lines and clinical samples

We are studying the role of PAX 6 in lung tumor cell lines by measuring tumor cell proliferation and migration after transfection of PAX6 into PAX6 negative cells and by knocking down PAX6 in PAX6 positive cells. Prognostic impact of PAX6 is investigated in lung cancer and sarcomas.

Contacts:

Ass. Professor. Yury Kiselev, yury.kiselev@uit.no, 77646295

Professor Lill-Tove Busund, lill.tove.busund@unn.no, 776 27221


Comparative studies of RNA and miRNA profiles in fresh frozen tumor tissue and FFPE material

The project is a pilot study for assessment of the expression profiles of RNA and miRNA from fresh frozen breast cancer specimens and formalin fixed/paraffin embedded samples (FFPE) from the same patients. The expression profiles will also be compared with blood samples from the same patients.

It is important to know the influence of different biological materials and different storing time and conditions of biological samples when comparing RNA/miRNA profiles.

Contacts:

Masterstudent Katinka Nøstdahl, kno005@post.uit.no, 77645482

Scientist Thomas Berg, thomas.berg@unn.no, 77645482

Professor Lill-Tove Busund, lill.tove.busund@unn.no, 776 27221