Biophysics

Biophysical characterization of kinases and of screening/design of inhibitors

•Expression and purification of kinase

•Development of assays to screen for potent inhibitors

•Activity assays for screening the inhibitory potential of new low molecular weight compounds

•Binding assay to determine binding constants Kd

•microscale theromphoresis

•Isotermal titration calorimetry

•surface plasmon resonace

•Cocrystallization and structural analysis of DYRK1B/Inhibitor complexes.