autumn 2014 BIO-8013 Applied molecular genetics - 10 ECTS
Course content
The aim of this course is to give a thorough introduction to molecular genetic methods used in modern biological research. The course will give a basis for advanced studies in different areas of biology and include practical exercises using both environmental and tissue specific samples. The students will isolate DNA and RNA from different samples and use Q-PCR for quantification of specific functional genes and transcripts. The laboratory work is running for two subsequent weeks and the results are discussed in plenum. The students have to write individual laboratory reports. The lectures will be closely connected to the practical problems to be solved in the laboratory. Selected articles from the scientific curriculum are topics for seminar presented by the students.Objectives of the course
Knowledge: Theory of gene and gene expression analyses Theory of microbial ecology Theory of nucleic acids extraction Critical consideration of scientific literature within the topics for the course How to write a laboratory report Critical assessment of own results Skills: Isolation of DNA and RNA from environmental samples Isolation of RNA from tissue cDNA synthesis and quality control Perform Q-PCR for quantification of gene abundance and expression Present objectives, methods and results. Planning molecular laboratory work Oral and written discussion of results Oral presentation of scientific topic General competence: Theory of environmental genetics Experimental design Molecular laboratory work Evaluation of methods Discussion of results Presentation of resultsTeaching methods
Lectures 8 hours, laboratory 40 hours within 2 weeks, seminars 10 hours.
Schedule:
BIO-8013 Applied molecular genetics has the same schedule as the master course BIO-3137. See BIO-3137 Applied Molecular GeneticsError rendering component
- About the course
- Campus: Tromsø |
- ECTS: 10
- Course code: BIO-8013
- Responsible unit
- Department of Arctic and Marine Biology