Dr. Sergei G. Kruglik from Sorbonne University, Laboratory Jean Perrin, and University Paris City, Laboratory MSC-Med, is a leading expert on Raman spectroscopy of biological nanoparticles. He will give this talk as part of his one-week visit to UiT.
Abstract:
The variety of bioparticles in the nanoscale domain attract nowadays an increasing attention of researches looking for both new fundamental knowledge and modern biomedical applications, including diseases diagnostics and therapies. These bioparticles include extracellular vesicles (EVs), protein aggregates, lipoproteins, bacteriophages and viruses, to name a few.
One of the major interests in my research lies in studying EVs, small specialized membranous bioparticles secreted from living cells and carrying specific biomolecular cargo. Recent general acceptance of the fact that EVs are intrinsically heterogenous not only by size, but also by biomolecular content, calls for new methods of investigation targeting single EVs, in addition to well established methods of bulk EVs characterization.
Raman Tweezers Microspectroscopy (RTM) is one of such investigation tools, combining Raman spectroscopy with particles’ optical trapping. In my talk, first I’ll provide a general description of the RTM technique which allows direct characterization of general biomolecular composition of single nanoscale bioparticle without using any external label.
Then, I’ll focus on our recent results when quantitative data derived from the RTM analysis of single EVs provide new information on biological processes with their participation. Special attention will be devoted to Raman spectra decomposition using Biomolecular Component Analysis for quantitative concentration measurements of major general classes of biomolecules: proteins, lipids, and nucleic acids.
Here is the list of recent relevant publications with my participation: