LacZymes - A structural view on enzymes involved in ß-lactam antibiotic resistance
LacZymes is a research group focusing on enzymes involved in antibiotic resistance. The enzymes breakdown the ß-lactam antibiotics and make the drug in active. The enzyme class is called ß-lactamases, and includes both metallo-ß-lactamases (MBLs) and serine-ß-lactamases (SBLs). The MBLs have zinc ions in the active site, whereas SBL have a serine residue in the active site.
Our long-term goal is to find new inhibitors for both ß-lactamase classes. If we find a new good inhibitor this inhibitor can be given in combination with a ß-lactam to prolong the lifetime of the currently used antibiotics. The group of ß-lactam antibiotics include penicillins, cephalosporins and carbapenems. The ß-lactam antibiotic have been on the marked for a long time, they are used, but worryingly many bacteria are resistant to these drugs. The most common resistance mechanism towards ß-lactam antibiotic is production of ß-lactamase enzymes.
Some input on Structural Bioinformatics.
Information on diagnistic routines of Antimicribial resistance are:
- Vazquez-Pertejo (2022) Diagnosis of Infectious Disease. MSD Manuals
- WHO (2017) A guide to aid the selection of diagnostic tests
- WHO (2018) report on Proof-of-principle antimicrobial resistance routine diagnostics surveillance project
We aim for solving ß-lactamases crystal structural at atomic resolution, characterize the same structures in biochemical assays in solution, and apply Surface plasmon resonance (SPR) to determine binding and release of inhibitors and substrates.
Last updated: 08.11.2023 13:43