Srijana Bastakoti
PhD Student
Job description
PhD research entitled "Identification of key determinants for Staphylococcus aureus throat colonization -: a transcriptomic approach"
- This Ph.D. project is all about “ Exploring Bacterial Transcriptomics ” in order to assess host-microbe interaction and present lead candidates for the targeted intervention of Staphylococcus aureus throat colonization and thereby prevent infection.
- Approach includes in vitro, ex vivo and bacterial co-culture establishment of the tonsillar cell line, and transcriptomics methodology including RNA sequencing (RNA-Seq) and qRT-PCR.
- Investigating transcriptomics responses of Staphylococcus aureus during exposure to human Thp-1 macrophages and host factors
- Metatranscriptomics will be performed directly on tonsillar tissue to investigate the in vivo expression system
- The transcriptome reflects the genes that are actively expressed at any given moment. This would enable us to identify bacterial determinants for S. aureus throat colonization in vitro and in vivo.
- Eventually, gaining an understanding of S. aureus adaptation and response to the host during colonization and infection.
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Publications outside Cristin
- Co-Author of Text book "Microbiology A Refresher" TU Solution Book For BSc First Year Tribhuvan University By Dynamic Publisher
- 2nd author paper: High-throughput sequencing of two European strains of tick-borne encephalitis virus (TBEV), Hochosterwitz and 1993 / 783- published in oct 2020 by Elsevier
- 1st author paper: Trichoderma species as Biocontrol Agent against Soil Borne Fungal Pathogens- published in dec 2017
Research interests
- Host-microbe interaction study
- Bacterial and viral genome, coding, and non-coding RNA,
- Exploring omics science
- Whole-genome sequencing (Illumina), RNA-seq, DNA-seq
- Bioinformatics, and data analysis
- Specialized in transcriptomics and the use of RNA sequencing technology in prokaryotic cells
- Exploring cutting-edge techniques, and how these can be deployed and exploited.
Teaching
- 2021 spring semester- MBI-1002- Biology
Carbohydrate and protein
cell signaling
lab teaching
- 2022 spring semester- MBI-1002